Measurement of uridine diphosphate glucuronic acid concentrations and synthesis in animal tissues.

In vitro reduction of zearalenone to β-zearalenol by rainbow trout (Oncorhynchus mykiss) hepatic microsomal and post-mitochondrial subfractions

Mycoestrogen zearalenone (ZEA) is found in human foods and animal feeds. Its estrogenic potencymainly depends on its biotransformation fate. The hepatic biotransformation of ZEA in rainbow trout wasinvestigated in this study. Various concentrations of ZEA were separately incubated with the hepaticmicrosomal and post-mitochondrial sub-fractions in the presence of NADPH, and the metabolites wered...

Control of colanic acid synthesis.

The nucleotide pools of certain mucoid, colanic acid-synthesizing strains of Escherichia coli, Salmonella typhimurium, and Aerobacter cloacae were examined, and in all cases the nucleotide sugars uridine-5'-diphosphate glucose (UDPG), uridine-5'-diphosphate galactose (UDPGal), guanosine-5'-diphosphate fucose (GDPF), and uridine-5'-diphosphate glucuronic acid (UDPGA) were detected. It is postula...

Enzymatic Formation of Uridine Diphosphoglucuronic

Many studies have shown that, although glucose is converted to glucuronides without cleavage of the carbon chain (2--S), free glucuronic acid functions poorly, if at all, as a precursor of glucuronic acid-containing compounds in living systems (e.g. phenol or alcohol glucuronides, mucopolysaccharides) (9-l 1). An important advance in elucidating the nature of the intermediates in the biosynthes...

The biochemistry and physiology of glucuronic acid: (a) The occurrence of conjugated glucuronic acids in the animal body. (b) Observations on the conjugation of d- and l-borneol.

Nucleotide activation of liver microsomal glucuronidation.

The classical studies of Dutton and Storey (1) have shown that liver microsomes catalyze the transfer of glucuronic acid from uridine diphosphate glucuronic acid to phenols. In an attempt to detect a reaction between uridine diphosphate glucuronic acid and uridine diphosphate N-acetylglucosamine, the effect of the latter on phenol glucuronidation was tested. It was expected that a reaction betw...